Clinical Physiology of Circulation

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


Interleukins 4 and 10 as factors of differentiation of subpopulations of blood monocytes in ischemic cardiomyopathy

Authors: S.P. Chumakova 1, D.A. Pogonchenkova 1, O.I. Urazova 1, M.V. Vins 1, V.M. Shipulin 2, A.S. Pryakhin 2, V.V. Novitskiy 1

Company:
1 Siberian State Medical University, Tomsk, 634050, Russian Federation
2 Cardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, 634012, Russian Federation

E-mail: Сведения доступны для зарегистрированных пользователей.

DOI: https://doi.org/10.24022/1814-6910-2020-17-1-52-57

UDC: 616.127

Link: Clinical Physiology of Blood Circulaiton. 2020; 17 (1): 52-57

Quote as: Chumakova S.P., Pogonchenkova D.A., Urazova O.I., Vins M.V., Shipulin V.M., Pryakhin A.S., Novitskiy V.V. Interleukins 4 and 10 as factors of differentiation of subpopulations of blood monocytes in ischemic cardiomyopathy. Clinical Physiology of Circulation. 2020; 17 (1): 52–7 (in Russ.). DOI: 10.24022/1814-6910-2020-17-1-52-57

Received / Accepted:  05.11.2019/09.12.2019

Full text:  

Abstract

Objective. To evaluate the ratio of the fractions of the classical, intermediate, non-classical and transitional monocytes in correlation with the concentration of interleukins 4 and 10 in the blood of patients with ischemic cardiomyopathy.

Material and methods. 18 patients with ischemic cardiomyopathy (17 men and 1 woman) aged 47–66 years with circulatory insufficiency of functional class II–III according on the classification of heart failure of the New York Heart Association examined. The group of control was made by 14 almost healthy donors comparable on gender and age to patients with an ischemic cardiomyopathy who don't have any diseases of cardiovascular system and also other systems of organs in an exacerbation stage. In blood of the patients with ischemic cardiomyopathy, the relative content of classical (CD14++CD16–), intermediate (CD14++CD16+), non-classical (CD14+CD16+) and transitional (CD14+CD16–) monocytes was assessed by flow cytometry and the concentration of interleukins (IL) 4 and 10 by enzyme-linked immunosorbent assay (ELISA).

Results. It was shown that the number of non-classical monocytes in the blood of patients with ischemic cardiomyopathy was 2 times lower than normal (5.05 [4.08; 6.58] % and 10.07 [9.34; 13.84] %, respectively, p=0.008), as well as the concentration of IL-4 (0.02 [0; 0.04] pg/ml and 0.15 [0.05; 0.63] pg/ml, respectively, p=0.039). The content of IL-10 in the blood was increased (30.05 [24.75; 33.50] pg/ml, p=0.042) and negatively correlated with the number of non-classical monocytes (r=–0.65; p=0.02). The number of classical monocytes in the blood of patients showed a tendency to decrease, and the proportion of intermediate monocytes, on the contrary, were slightly higher than in healthy individuals. The relative content of transitional monocytes in the blood was comparable with that of healthy donors.

Conclusions.Thus, the subpopulation composition of blood monocytes in patients with ischemic cardiomyopathy is characterized by a deficiency of the fraction of non-classical monocytes with protective properties against endothelium, and interleukin-4 in the blood with an excess of IL-10 and certain increase the number of intermediate cells with ability to cooperate with T-lymphocytes, which predisposes to common atheromatosis of small coronary arteries and diffuse hypoxic myocardial lesions in ischemic cardiomyopathy.

References

  1. Richardson P., McKenna W., Bristow M., Maisch В., Mautner В., O'Connell J. et al. Report of the 1995 World Health Organization/International Society and Federation of Cardiology Task Force on the Definition and Classification of cardiomyopathies. Circulation. 1996; 93 (5): 841–2.
  2. Осовская Н.Ю. Ишемическая кардиомиопатия: терминология, эпидемиология, патофизиология, диагностика, подходы к лечению. Новости медицины и фармации. 2011; 359: 16–9. [Osovskaya N.Yu. Ischemic cardiomyopathy: terminology, epidemiology, pathophysiology, diagnosis, therapeutic approach. News of Medicine and Pharmacy. 2011; 359: 16–9 (in Russ.).]
  3. Bartekova M., Radosinska J., Jelemensky M., Dhalla N.S. Role of cytokines and inflammation in heart function during health and disease. Heart Fail. Rev. 2018; 23 (5): 733–58. DOI: 10.1007/s10741-0189716-x
  4. Лежнёв А.А., Шипулин В.М., Кривощеков Е.В. Послеоперационное ремоделирование левого желудочка. Клинические и морфологические аспекты. LAP Lambert Academic Publishing; 2012. [Lezhnev A.A., Shipulin V.M., Krivoshchekov E.V. Postoperative left ventricular remodeling. Clinical and morphological aspects. LAP Lambert Academic Publishing; 2012 (in Russ.).]
  5. Guddeti R.R., Matsuo Y., Matsuzawa Y., Kushwaha S.S., Lerman A. Ischemic cardiomyopathy is associated with coronary plaque progression and higher event rate in patients after cardiac transplantation. J. Am. Heart Ass. 2014; 3 (4): 10–91. DOI: 10.1161/ JAHA.114.001091
  6. Коваленко В.Н., Гавриш А.С. Ишемическая кардиомиопатия: нейрогуморальные и иммуновоспалительные аспекты пато- и морфогенеза. Украинский кардиологический журнал. 2013; 4: 14–20. [Kovalenko V.N., Gavrish A.S. Ischemic cardiomyopathy: neurohumoral and immuno-logical aspects of patho- and morphogenesis. Ukrainian Journal of Cardiology. 2013; 4: 14–20 (in Russ.).]
  7. Ziegler-Heitbrock L. The CD14+CD16+ blood monocytes: their role infection and inflammation. J. Leuk. Biol. 2007; 3: 584–92. DOI: 10.1189/jlb.0806510
  8. Ziegler-Heitbrock L., Hofer T.P.J. Toward a refined definition of monocyte subsets. Front. Immunol. 2013; 4: 23. DOI: 10.3389/fimmu.2013.00023
  9. Матвеева В.Г., Головкин А.С., Кудрявцев И.В., Григорьев Е.В., Чернова М.Н. Динамика CD14+CD16+ субпопуляций моноцитов при неосложненном системном воспалительном ответе в периоперационном периоде коронарного шунтирования. Медицинская иммунология. 2012; 14 (4–5): 391–8. DOI: 10.15789/1563-0625-2012-4-5-391-398 [Matveeva V.G., Golovkin A.S., Kudryavtsev I.V., Grigoriev E.V., Chernova M.N. Dynamics of CD14+CD16+ monocyte subpopulations in complication-free systemic inflammatory response following coronary artery bypass graft surgery. Medical Immunology (Russia). 2012; 14 (4–5): 391–8 (in Russ.). DOI: 10.15789/1563-0625-2012-4-5-391-398 ]
  10. Казаков В.А., Шипулин В.М., Лежнев А.А., Козлов Б.Н., Кривощеков Е.В., Суходоло И.В., Гутор С.С. Клинико-морфологические параллели послеоперационного ремоделирования левого желудочка. Патология кровообращения и кардиохирургия. 2009; 4: 23–7. [Kazakov V.A., Shipulin V.M., Lezhnev A.A., Kozlov B.N., Krivoshchekov E.V., Sukhodolo I.V., Gutor S.S. The clinic and morphological parallel postoperative remodeling of the left ventricle. Circulation Pathology and Cardiac Surgery. 2009; 4: 23–7 (inRuss.).]
  11. Казаков В.А., Суходоло В.И., Шипулин В.М. Ультраструктурные аспекты постинфарктного ремоделирования левого желудочка. Сибирский медицинский журнал. 2009; 4: 6–11. [Kazakov V.A., Sukhodolo V.I., Shipulin V.M. Ultrastructural aspects of postinfarction remodeling of the left ventricle. Siberian Medical Journal. 2009; 4: 6–11 (in Russ.).]
  12.  Italiani P., Boraschi D. From monocytes to M1/M2 macrophages: phenotypical vs. functional differentiation. Front. Immunol. 2014; 5: 514. DOI: 10.3389/ fimmu.2014.00514
  13. Dutta P., Nahrendorf M. Monocytes in myocardial infarction. Arterioscler. Thromb. Vasc. Biol. 2015; 35: 1066–70. DOI: 10.1161/ATVBAHA.114.304652
  14. Barisionea C., Garibaldia S., Ghigliottia G., Fabbia P., Altieria P., Casalea M. et al. CD14CD16 monocyte subset levels in heart failure patients. Disease Marker. 2010; 28 (2): 115–24. DOI: 10.3233/DMA-2010-0691
  15. Лилли Л.С. (ред.) Патофизиология сердечно-сосудистой системы. Пер. с англ. 4-е изд. М.: Бином, Лаборатория знаний; 2016. [Lilly L.S. (Ed.) Pathophysiology of the cardiovascular system. Transl. from Engl. 4th ed. Moscow: Binom, Laboratoriya znaniy; 2016 (in Russ.).]
  16. Fadini G.P., Simoni F., Cappellari R., Previato L., Avogaro A. Pro-inflammatory monocyte-macrophage polarization imbalance in human hypercholesterolemia and atherosclerosis. Atherosclerosis. 2014; 237 (2): 805–8. DOI: 10.1016/j.atherosclerosis.2014.10.106
  17. Jaipersad A.S., Shantsila A., Lip G.Y.H., Shantsila E. Expression of monocyte subsets and angiogenic markers in relation to carotid plaque neovascularization in patients with pre-existing coronary artery disease and carotid stenosis. Ann. Med. 2014; 46 (7): 530–8. DOI: 10.3109/07853890.2014.931101
  18. Esper R.J. Hypertension arterial mechanics and other issues. Revista Argent. Cardiol. 2011; 79 (4): 13.

About Authors

  • Svetlana P. Chumakova, Dr. Мed. Sc., Professor of the Pathophysiology Chair; orcid.org/0000-0003-3468-6154
  • Dar’ya A. Pogonchenkova, Applicant of the Pathophysiology Chair; orcid.org/0000-0002-5903-3662
  • Ol’ga I. Urazova, Dr. Med. Sc., Professor, Corresponding Member of RAS, Chief of Pathophysiology Chair; orcid.org/0000-0002-9457-8879
  • Mariya V. Vins, Assistant of the Pathophysiology Chair Vladimir M. Shipulin, Dr. Med. Sc., Professor, Head of the Cardiovascular Surgery Department
  • Andrey S. Pryakhin, Postgraduate; orcid.org/0000-0003-0532-8091
  • Vyacheslav V. Novitskiy, Dr. Мed. Sc., Professor, Academician of RAS; orcid.org/0000-0002-9577-8370


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