Clinical Physiology of Circulation

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


Studies on the interaction of heparin with de-endothelialized arterial surface

Authors: V.M. Yunusov 1, V.V. Plechev 2, R.I. Izhbul'din 1, S.S. Ostakhov 3, A.N. Kislitsyn 1

Company:
1 Republican Cardiologic Dispensary, ul. St. Kuvykina, 96, Ufa, 450106, Russia;
2 Chair of Hospital Surgery of Bashkirsky State Medical University, ul. Lenina, 3, Ufa, 450000, Russia;
3 Institute of Organic Chemistry of the Ufa Scientific Center of the Russian Academy of Sciences, pr. Oktyabrya, 71, Ufa, 450054, Russia

E-mail: Сведения доступны для зарегистрированных пользователей.

Link: Clinical Physiology of Blood Circulaiton. 2013; (): -

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Abstract

Objective of the research – is to study the possibilities of intracoronary heparin delivery after coronary artery endarterectomy procedure.

Material and methods. The processes of adsorption of heparin by the tissue of aorta were studied using the spectral luminescence method. The anticoagulant activity of heparin was estimated on coagulometer «Act plus» (Medtronic, USA).

Results. The method of quenching of tryptophan fluorescence by heparin was studied and the stability constant of its complexes with amino acids was determined. Consistent patterns of adsorption of heparin by the tissue of aorta with and without endothelium under multiple conditions were detected. Anticoagulant properties of the complex heparin – aromatic amino acids were examined. It was proved that heparin has high anticoagulant activity in the state bound to amino acids.

Conclusion. The possibility of intracoronary heparin delivery after coronary artery endarterectomy was proved experimentally whereas anticoagulant activity of this therapeutic agent is not reduced.

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