Clinical Physiology of Circulation

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


Interleukins 4 and 10 as factors of differentiation of subpopulations of blood monocytes in ischemic cardiomyopathy

Authors: S.P. Chumakova 1, D.A. Pogonchenkova 1, O.I. Urazova 1, M.V. Vins 1, V.M. Shipulin 2, A.S. Pryakhin 2, V.V. Novitskiy 1

Company:
1 Siberian State Medical University, Tomsk, 634050, Russian Federation
2 Cardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, 634012, Russian Federation

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DOI: https://doi.org/10.24022/1814-6910-2020-17-1-52-57

UDC: 616.127

Link: Clinical Physiology of Blood Circulaiton. 2020; 17 (1): 52-57

Quote as: Chumakova S.P., Pogonchenkova D.A., Urazova O.I., Vins M.V., Shipulin V.M., Pryakhin A.S., Novitskiy V.V. Interleukins 4 and 10 as factors of differentiation of subpopulations of blood monocytes in ischemic cardiomyopathy. Clinical Physiology of Circulation. 2020; 17 (1): 52–7 (in Russ.). DOI: 10.24022/1814-6910-2020-17-1-52-57

Received / Accepted:  05.11.2019/09.12.2019

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Abstract

Objective. To evaluate the ratio of the fractions of the classical, intermediate, non-classical and transitional monocytes in correlation with the concentration of interleukins 4 and 10 in the blood of patients with ischemic cardiomyopathy.

Material and methods. 18 patients with ischemic cardiomyopathy (17 men and 1 woman) aged 47–66 years with circulatory insufficiency of functional class II–III according on the classification of heart failure of the New York Heart Association examined. The group of control was made by 14 almost healthy donors comparable on gender and age to patients with an ischemic cardiomyopathy who don't have any diseases of cardiovascular system and also other systems of organs in an exacerbation stage. In blood of the patients with ischemic cardiomyopathy, the relative content of classical (CD14++CD16–), intermediate (CD14++CD16+), non-classical (CD14+CD16+) and transitional (CD14+CD16–) monocytes was assessed by flow cytometry and the concentration of interleukins (IL) 4 and 10 by enzyme-linked immunosorbent assay (ELISA).

Results. It was shown that the number of non-classical monocytes in the blood of patients with ischemic cardiomyopathy was 2 times lower than normal (5.05 [4.08; 6.58] % and 10.07 [9.34; 13.84] %, respectively, p=0.008), as well as the concentration of IL-4 (0.02 [0; 0.04] pg/ml and 0.15 [0.05; 0.63] pg/ml, respectively, p=0.039). The content of IL-10 in the blood was increased (30.05 [24.75; 33.50] pg/ml, p=0.042) and negatively correlated with the number of non-classical monocytes (r=–0.65; p=0.02). The number of classical monocytes in the blood of patients showed a tendency to decrease, and the proportion of intermediate monocytes, on the contrary, were slightly higher than in healthy individuals. The relative content of transitional monocytes in the blood was comparable with that of healthy donors.

Conclusions.Thus, the subpopulation composition of blood monocytes in patients with ischemic cardiomyopathy is characterized by a deficiency of the fraction of non-classical monocytes with protective properties against endothelium, and interleukin-4 in the blood with an excess of IL-10 and certain increase the number of intermediate cells with ability to cooperate with T-lymphocytes, which predisposes to common atheromatosis of small coronary arteries and diffuse hypoxic myocardial lesions in ischemic cardiomyopathy.

About Authors

  • Svetlana P. Chumakova, Dr. Мed. Sc., Professor of the Pathophysiology Chair; orcid.org/0000-0003-3468-6154
  • Dar’ya A. Pogonchenkova, Applicant of the Pathophysiology Chair; orcid.org/0000-0002-5903-3662
  • Ol’ga I. Urazova, Dr. Med. Sc., Professor, Corresponding Member of RAS, Chief of Pathophysiology Chair; orcid.org/0000-0002-9457-8879
  • Mariya V. Vins, Assistant of the Pathophysiology Chair Vladimir M. Shipulin, Dr. Med. Sc., Professor, Head of the Cardiovascular Surgery Department
  • Andrey S. Pryakhin, Postgraduate; orcid.org/0000-0003-0532-8091
  • Vyacheslav V. Novitskiy, Dr. Мed. Sc., Professor, Academician of RAS; orcid.org/0000-0002-9577-8370


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