Clinical Physiology of Circulation

ISSN 1814-6910 (Print)
ISSN 2410-9134 (Online)

 

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


Issue's catalogue ➤ КФК. 2020; 17 (1): 5-69 ➤ Новые перспективы исследования гемостатических покрытий на основе хитозана in vivo и in vitro

New prospects of research of hemostatic dressings based on chitosan in vivo and in vitro

Authors: G.G. Belozerskaya 1, A.P. Momot 2, M.V. Pykhteeva 3, V.А. Kabak 1, О.E. Nevedrova 1, D.Yu. Bychichko 1, А.R. Lempert 1, L.S. Malykhina 1, D.A. Momot 5, Е.M. Golubev 1, Т.I. Shirokova 1, M.S. Mironov 1, L.V. Akopyan 4

Company:
1 National Medical Research Center for Hematology, Moscow, 125167, Russian Federation
2 Altai Branch of the National Medical Research Center for Hematology, Barnaul, 656045, Russian Federation
3 Regional Clinical Hospital, Barnaul, 656045, Russian Federation
4 A.I. Yevdokimov Moscow State University of Medicine and Dentistry, Moscow, 127473, Russian Federation
5 Altai State Medical University, Barnaul, 656038, Russian Federation

E-mail: Сведения доступны для зарегистрированных пользователей.

DOI: https://doi.org/10.24022/1814-6910-2020-17-1-58-69

UDC: 616.12-089-092.9:620.3

Link: Clinical Physiology of Blood Circulaiton. 2020; 17 (1): 58-69

Quote as: Belozerskaya G.G., Momot A.P., Pykhteeva M.V., Kabak V.A., Nevedrova O.E., Bychichko D.Yu., Lempert A.R., Malykhina L.S., Momot D.A., Golubev E.M., Shirokova T.I., Mironov M.S., Akopyan L.V. New prospects of research of hemostatic dressings based on chitosan in vivo and in vitro. Clinical Physiology of Circulation. 2020; 17 (1): 58–69 (in Russ.). DOI: 10.24022/1814-6910-2020-17-1-58-69

Keywords:  hemostasis hemostatics of local action bleeding rabbits natural polymers thrombin generation test thromboelastometry chitosan

Received / Accepted:  07.11.2019/26.11.2019

Download
Full text:  

Abstract

Objective. To conduct an experimental assessment of the hemostatic activity of local dressings in the form of a sponge based on chitosan solutions in vivo and in vitro.

Material and methods. For the production of the investigated dressings were used chitosan solutions with a mass fraction of 0.5, 1.0, 1.5 and 2.0% using 0.5% acetic or 0,5% lactic acids as solvents. On the basis of these solutions experimental samples of dressings in the form of a sponge were made. Hemostatic activity was determined by measuring of the time to stop bleeding and blood loss. The influence of the interaction of the contact surface of the dressings in the form of a sponge with blood was assessed by the results of counting the number of platelets and determining the level of fibrinogen in the blood plasma. An integral assessment of the hemostasis system was performed using thromboelastometry of blood stabilized with sodium citrate and thrombin generation test in blood plasma.

Results. High hemostatic activity in experiments in vivo had local sponge-based dressings based on 0.5 and 2.0% chitosan solutions in 0.5% acetic acid (67.65±24.23% and 65.65±9.68% respectively). The results of in vitrostudies of these dressings confirm the acceleration of the formation of a primary thrombus during their interaction with blood. Dressings based on all chitosan solutions in 0.5% lactic acid and 1,0 and 1.5% solutions of chitosan in 0.5% acetic acid did not show hemostatic activity in in vivo experiments and practically did not change the parameters of hemostasis in vitro.

Conclusion. The interconnection between the values of the hemostatic activity of wound dressings based on chitosan solutions in acetic acid in vivo during their local application to the wound surface of the rabbit's liver and researches of the system of hemostasis in vitro was revealed. Joint carrying out researches on studying of a system of a hemostasis both in vivo, and in vitro is represented the perspective direction for assessment of interrelation of a structural state and the mechanism of action of new hemostatics.

References

  1. Липатов В.А., Ершов М.П., Сотников К.А., Ушанов А.А., Новикова Н.В., Константинова Ю.Е. Современные тенденции применения локальных аппликационных кровоостанавливающих средств. Научный электронный журнал «Innova». 2016; 2 (3): 64–9. DOI: 10.21626/innova/2016.2/07 [Lipatov V.A., Ershov M.P., Sotnikov K.A.,Ushanov A.A., Novikova N.V., Konstantinova Yu.E. Current trends in the use of local application of hemostatic agents. Scietific Electronic Journal “Innova”. 2016; 2 (3): 64–9. DOI: 10.21626/innova/2016.2/07 (in Russ.).]
  2. Bennett B.L. Bleeding control using hemostatic dressings: lessons learned. Wild. Environment. Med. 2017; 28 (2S): S39–49. DOI: 10.1016/j.wem.2016.12.005
  3. Kiruthika P., Dhanraj M., Jain A.R. Effectiveness of local hemostatic agents following dental extraction: a systematic review. J. Advanc. Pharm. Educ. Res. 2017; 7 (1): 5–8.
  4. Yang X., Liu W., Li N., Wang M., Liang B., Ullah I. et al. Design and development of polysaccharide hemostatic materials and their hemostatic mechanism. Biomat. Sc. 2017; 5 (12): 2357–68. DOI: 10.1039/ C7BM00554G
  5. Гурин А.Н., Комлев В.С., Федотов А.Ю., Берковский А.Л., Мамонов В.Е., Григорьян А.С. Сравнительная характеристика материалов на основе хитозана, альгината и фибрина в комплексе с β-трикальцийфосфатом для остеопластики (экспериментально-морфологическое исследование). Стоматология. 2014; 93 (1): 4–10. [Gurin A.N., Komlev V.S., Fedotov A.Yu., Berkovskiy A.L., Mamonov V.E., Grigor'yan A.S. Comparative study of osteoplastic materials based on chitosan, alginate or fibrin with tricalcium phosphate. Stomatology. 2014; 93 (1): 4–10 (in Russ.).]
  6. Khan M.A., Mujahid M. A review on recent advances in chitosan based composite for hemostatic dressings. Intern. J. Biol. Macromol. 2019; 124: 138–47.
  7. Pan M., Tang Z., Tu J., Wang Z., Chen Q., Xiao R. et al. Porous chitosan microspheres containing zinc ion for enhanced thrombosis and hemostasis. Mat. Sc. Engineer. C. 2018; 85: 27–36. DOI: 10.1016/j.msec. 2017.12.015
  8. Sims K., Montgomery H.R., Dituro P., Kheirabadi B.S., Butler F.K. Management of external hemorrhage in tactical combat casualty care: the adjunctive use of XStatTM compressed hemostatic sponges: TCCC Guidelines Change 15-03. J. Spec. Oper. Med. Peer Rev. J. SOF Med. Prof. 2016; 16 (1): 19–28.
  9.  Абзаева К.А., Зеленков Л.Е. Современные локальные гемостатики и уникальные представители их нового поколения. Известия Академии наук. Серия химическая. 2015; 6: 1233–9. [Abzaeva К.А., Zelenkov L.Е. Modern local hemostatics and unique representatives of their new generation. Russian Chemical Bulletin. 2015; 6: 1233–9 (in Russ.).]
  10. Adamiak Z., Bukowiecka D., Jastrze ,bski P., Jałyn ′skiM., Holak P., Głodek J. et al. The effectiveness of modified seton and modified combat gauze in controlling severe hemorrhaging during operations of uniformed services. Pol. J. Vet. Sc. 2016; 19 (3): 503–7. DOI: 10.1515/pjvs-2016-0063
  11. Chan L.W., Kim C.H., Wang X., Pun S.H., White N.J., Kim T.H. PolySTAT-modified chitosan gauzes for improved hemostasis in external hemorrhage. Acta Biomat. 2016; 31: 178–85. DOI: 10.1016/j.actbio. 2015.11.017
  12. Saporito F., Sandri G., Rossi S., Bonferoni M.C., Riva F., Malavasi L. et al. Freeze dried chitosan acetate dressings with glycosaminoglycans and traxenamic acid. Carbohydr. Polym. 2018; 184: 408–17. DOI: 10.1016/ j.carbpol.2017.12.066
  13. Миронов А.Н. (ред.). Руководство по проведению доклинических исследований лекарственных средств. Часть первая. М.: Гриф и К; 2012. [Mironov А.N. (Ed.). A guide to preclinical drug research. Part 1th. Moscow: Grif and К; 2012 (in Russ.).]
  14. Hemker H.C., Al Dieri R., Be ′guin S. Thrombin generation assays: accruing clinical relevance. Curr. Opin. Hematol. 2004; 11: 170–5. DOI: 10.1097/01.moh. 0000130314.33410.d7
  15. Белозерская Г.Г., Момот А.П., Пыхтеева М.В., Белозеров Д.Е., Бычичко Д.Ю., Неведрова О.Е. и др. Сравнительная оценка гемостатических свойств локальных покрытий на основе каппа-каррагинана in vitro. Клиническая физиология кровообращения. 2019; 16 (2): 148–57. DOI: 10.24022/1814-6910-201916-2-148-157 [Belozerskaya G.G., Momot A.P., Pykhteeva M.V., Belozerov D.E., Bychichko D.Yu., Nevedrova O.E. et al. Comparative evaluation of the hemostatic properties of local coatings based on kappa-carrageenan in vitro. Clinical Physiology of Circulation. 2019; 16 (2): 148–57 (in Russ.). DOI: 10.24022/1814-6910-2019-16-2-148-157 ]

About Authors

  • Galina G. Belozerskaya, Dr. Med. Sc., Head of Laboratory of Pathology and Pharmacology of Hemostasis; orcid.org/0000-0001-8620-153X
  • Andrey P. Momot, Dr. Med. Sc., Professor, Director of the Altai Branch; orcid.org/0000-0002-8413-5484
  • Marina V. Pykhteeva, Doctor-laboratory assistant; orcid.org/0000-0003-4810-2922
  • Valeriy A. Kabak, Manager; orcid.org/0000-0002-3851-7510
  • Ol'ga E. Nevedrova, Cand. Biol. Sc., Senior Researcher; orcid.org/0000-0001-9752-6647
  • Dmitriy Yu. Bychichko, Doctor-Biochemist, Junior Researcher; orcid.org/0000-0003-1585-4415
  • Asaf R. Lempert, Trainee-Researcher; orcid.org/0000-0002-6576-5712
  • Larisa S. Malykhina, Cand. Biol. Sc., Senior Researcher; orcid.org/0000-0002-6231-0069
  • Dmitriy A. Momot, Assistant of Chair of Faculty Therapy; orcid.org/0000-0002-7692-5048
  • Evgeniy M. Golubev, Head of Experimental and Production Department of Deep Processing of Plasma; orcid.org/0000-0002-5405-8270
  • Tat'yana I. Shirokova, Assistant Head of Experimental and Production Department of Deep Processing of Plasma; orcid.org/0000-0002-2543-8071
  • Maksim S. Mironov, Laboratory Assistant; orcid.org/0000-0001-5230-7424
  • Lyudmila V. Akopyan, Cand. Med. Sc., Assistant of Chair of Otorhinolaryngology; orcid.org/0000-0002-4328-0001

 If you found mistakes, select text and press Alt+A