Clinical Physiology of Circulation

ISSN 1814-6910 (Print)
ISSN 2410-9134 (Online)

 

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


Issue's catalogue ➤ КФК. 2021; 18 (2): 93-180 ➤ Покрытия для локального гемостаза с микрои наночастицами

Dressings with micro- and nanoparticles for local hemostasis

Authors: Belozerskaya G.G., Momot A.P., Lempert А.R., Bychichko D.Yu., Kabak V.А., Nevedrova О.E., Logvinova Yu.S., Mironov M.S., Sivkov A.A., Shanenkov I.I., Golubev Е.M., Shirokova Т.I., Pykhteeva M.V.

Company:
1 National Medical Research Center for Hematology, Moscow, 125167, Russian Federation
2 Altai Branch of the National Medical Research Center for Hematology, Barnaul, 656024, Russian Federation
3 Regional Clinical Hospital, Barnaul, 656024, Russian Federation
4 Tomsk Polytechnic University, Tomsk, 634050, Russian Federation

E-mail: Сведения доступны для зарегистрированных пользователей.

DOI: https://doi.org/10.24022/1814-6910-2021-18-2-172-180

UDC: 616.15

Link: Clinical Physiology of Blood Circulaiton. 2021; 2 (18): 172-180

Quote as: Belozerskaya G.G., Momot A.P., Lempert А.R., Bychichko D.Yu., Kabak V.А., Nevedrova О.E., Logvinova Yu.S., Mironov M.S., Sivkov A.A., Shanenkov I.I., Golubev Е.M., Shirokova Т.I., Pykhteeva M.V. Dressings with micro- and nanoparticles for local hemostasis. Clinical Physiology of Circulation. 2021; 18 (2): 172–80 (in Russ.). DOI: 10.24022/1814-6910-2021-18-2-172-180

Keywords:  sodium alginate hemostasis hemostatics of local action bleeding nanoparticles natural polymers

Received / Accepted:  18.03.2021 / 16.04.2021

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Abstract

Objective. Creation of sponge’s form local dressings based on sodium alginate with nanoparticles of iron oxides integration. Assessment of hemostatic activity of created dressings in vivo and in vitro.

Material and methods. For the production of the investigated dressings solutions of sodium alginate were used. The mixtures of ultradispersed powders of micro and nanoparticles of iron oxides in various phase were added to the solutions. Hemostatic activity was determined by measuring of the time to stop bleeding and blood loss. The effect of the interaction of the contact surface sponge’s form dressings with blood was assessed by the counting the number of platelets, determining the level of fibrinogen, indicators of thromboelastometry and thrombin generation test.

Results. Sponge’s form dressings based on 2,0% sodium alginate solutions with iron oxide nanoparticles integrated have high hemostatic activity, enhance the reaction of the primary clot’s formation and enhance the thrombin generation reaction. The dependence of hemostatic activity on the polymer concentration, quantitative composition of nanoparticles and distribution of nanoparticles in the structure of the samples was revealed.

Conclusion. Analysis of the hemostatic properties of local wound dressings in the form of a sponge based on sodium alginate solutions in vivo and in vitro showed that the addition of a mixture of ultradispersed powders of micro- and nanoparticles of iron oxides with the prevalence of the epsilon phase led to an increase in the hemostatic properties of the dressings.

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About Authors

  • Galina G. Belozerskaya, Dr. Med. Sc., Head of Laboratory of Pathology and Pharmacology of Hemostasis; ORCID
  • Andrey P. Momot, Dr. Med. Sc., Professor, Director; ORCID
  • Asaf R. Lempert, Cand. Med. Sc., Trainee-Researcher of Laboratory of Pathology and Pharmacology of a Hemostasis; ORCID
  • Dmitriy Yu. Bychichko, Junior Researcher, Doctor-Biochemist; ORCID
  • Valeriy A. Kabak, Manager of Laboratory of Pathology and Pharmacology of a Hemostasis; ORCID
  • Ol’ga E. Nevedrova, Cand. Biol. Sc., Senior Researcher; ORCID
  • Yulia S. Logvinova, Cand. Med. Sc., Researcher, Doctor-Biochemist; ORCID
  • Maksim S. Mironov, Laboratory Assistant; ORCID
  • Aleksandr A. Sivkov, Dr. Tech. Sc., Professor; ORCID
  • Ivan I. Shanenkov, Cand. Tech. Sc., Associate Professor; ORCID
  • ГEvgeniy M. Golubev, Head of Experimental and Production Department of Deep Processing of Plasma; ORCID
  • Tat’yana I. Shirokova, Deputy Head of Experimental and Production Department of Deep Processing of Plasma; ORCID
  • Marina V. Pykhteeva, Doctor-Laboratory Assistant of the Laboratory of Pathology of Hemostasis; ORCID

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