Clinical Physiology of Circulation

ISSN 1814-6910 (Print)
ISSN 2410-9134 (Online)

 

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


Issue's catalogue ➤ КФК. 2024; 21 (1): 1-88 ➤ Влияние оксида азота и молекулярного водорода на окислительные, антиоксидантные и агрегационные показатели эритроцитов при операциях в условиях искусственного кровообращения

The effect of nitric oxide and molecular hydrogen on oxidative, antioxidant and aggregation indicators of erythrocytes during cardiac surgery with cardiopulmonary bypass

Authors: Deryugina A.V.1, Pichugin V.V.23, Danilova D.A.1, Trofimov R.D.23, Starshov A.S.23, Doronina M.A.1, Domnin S.E.3, Taranov E.V.23, Zhilyaev S.A.3, Brichkin Yu.D.3

Company:
1 Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russian Federation
2 Volga Region Research Medical University, Nizhny Novgorod, Russian Federation
3 Research Institute – Specialized Cardiac Surgical Clinical Hospital named after academician B.A. Korolev, Nizhny Novgorod, Russian Federation

E-mail: Сведения доступны для зарегистрированных пользователей.

DOI: https://doi.org/10.24022/1814-6910-2024-21-1-45-55

UDC: 612.111.31:616.12-089

Link: Clinical Physiology of Blood Circulaiton. 2024; 21 (1): 56-68

Quote as: Deryugina A.V., Pichugin V.V., Danilova D.A., Trofimov R.D., Starshov A.S., Doronina M.A., Domnin S.E., Taranov E.V., Zhilyaev S.A., Brichkin Yu.D. The effect of nitric oxide and molecular hydrogen on oxidative, antioxidant and aggregation indicators of erythrocytes during cardiac surgery with cardiopulmonary bypass. Clinical Physiology of Circulation. 2024; 21 (1): 45–55 (in Russ.). DOI: 10.24022/1814-6910-2024-21-1-45-55

Keywords:  nitric oxide molecular hydrogen operations with cardiopulmonary bypass erythrocytes

Received / Accepted:  19.01.2024 / 07.03.2024

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Abstract

Objective. To study the oxidative potential, antioxidant properties and aggregation of erythrocytes under the action of molecular hydrogen and nitric oxide during cardiopulmonary bypass (CPB) in cardiac surgery patients.

Material and methods. The study included 44 patients who underwent heart valve surgeries and combined interventions under CPB. The patients were divided into 4 groups. Group 1 – control; group 2 – patients with isolated supply of nitric oxide (NO) into the extracorporeal circuit; group 3 – with supply of molecular hydrogen (H2); group 4 – with combined supply of NO and H2. The concentration of malondialdehyde (MDA), catalase activity (CA) in erythrocytes and erythrocyte aggregation were studied during and after surgery.

Results. The MDA concentration and erythrocyte aggregation increased and СА decreased during CPB at all stages of the study in the control group of patients. NO decreased the MDA concentration and increased CA compared to the control throughout the operation. These indicators were restored to the initial level by the end of the operation under the influence of NO. Aggregation decreased by 1 day after the operation under the influence of NO. H2 caused more significant changes compared to NO. The MDA concentration under the action of H2 was lower than the initial level by the end of surgery and CA and was up to 1 day after the operation. The dynamics MDA concentration and CA under the combined action of NO and H2 were comparable to the action of NO at the stages of the operation. The combined effect of gases was more pronounced on the 1st day after the operation, which was combined with the maximum disaggregating effect compared to other groups

Conclusion. Thus the use of NO, H2 and combination of NO with H2 caused a decrease in oxidative processes and erythrocyte aggregation, expressed during CPB.

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About Authors

  • Anna V. Deryugina, Dr. Biol. Sci.; Associate Professor, Chief of Chair; ORCID
  • Vladimir V. Pichugin, Dr. Med. Sci., Professor, Anesthesiologist-Intensivist; ORCID
  • Darya A. Danilova, Senior Lecturer; ORCID
  • Roman D. Trofimov, Postgraduate; ORCID
  • Artem S. Starshov, Postgraduate; ORCID
  • Margarita A. Doronina, Undergraduate; ORCID
  • Stepan E. Domnin, Cand. Med. Sci., Anesthesiologist-Intensivist; ORCID
  • Evgeniy V. Taranov, Anesthesiologist-Intensivist; ORCID
  • Sergey A. Zhilyaev, Anesthesiologist-Intensivist; ORCID
  • Yuriy D. Brichkin, Dr. Med. Sci., Professor; ORCID

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