Clinical Physiology of Circulation

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


Dressings with micro- and nanoparticles for local hemostasis

Authors: Belozerskaya G.G., Momot A.P., Lempert А.R., Bychichko D.Yu., Kabak V.А., Nevedrova О.E., Logvinova Yu.S., Mironov M.S., Sivkov A.A., Shanenkov I.I., Golubev Е.M., Shirokova Т.I., Pykhteeva M.V.

Company:
1 National Medical Research Center for Hematology, Moscow, 125167, Russian Federation
2 Altai Branch of the National Medical Research Center for Hematology, Barnaul, 656024, Russian Federation
3 Regional Clinical Hospital, Barnaul, 656024, Russian Federation
4 Tomsk Polytechnic University, Tomsk, 634050, Russian Federation

E-mail: Сведения доступны для зарегистрированных пользователей.

DOI: https://doi.org/10.24022/1814-6910-2021-18-2-172-180

UDC: 616.15

Link: Clinical Physiology of Blood Circulaiton. 2021; 2 (18): 172-180

Quote as: Belozerskaya G.G., Momot A.P., Lempert А.R., Bychichko D.Yu., Kabak V.А., Nevedrova О.E., Logvinova Yu.S., Mironov M.S., Sivkov A.A., Shanenkov I.I., Golubev Е.M., Shirokova Т.I., Pykhteeva M.V. Dressings with micro- and nanoparticles for local hemostasis. Clinical Physiology of Circulation. 2021; 18 (2): 172–80 (in Russ.). DOI: 10.24022/1814-6910-2021-18-2-172-180

Received / Accepted:  18.03.2021 / 16.04.2021

Full text:  

Abstract

Objective. Creation of sponge’s form local dressings based on sodium alginate with nanoparticles of iron oxides integration. Assessment of hemostatic activity of created dressings in vivo and in vitro.

Material and methods. For the production of the investigated dressings solutions of sodium alginate were used. The mixtures of ultradispersed powders of micro and nanoparticles of iron oxides in various phase were added to the solutions. Hemostatic activity was determined by measuring of the time to stop bleeding and blood loss. The effect of the interaction of the contact surface sponge’s form dressings with blood was assessed by the counting the number of platelets, determining the level of fibrinogen, indicators of thromboelastometry and thrombin generation test.

Results. Sponge’s form dressings based on 2,0% sodium alginate solutions with iron oxide nanoparticles integrated have high hemostatic activity, enhance the reaction of the primary clot’s formation and enhance the thrombin generation reaction. The dependence of hemostatic activity on the polymer concentration, quantitative composition of nanoparticles and distribution of nanoparticles in the structure of the samples was revealed.

Conclusion. Analysis of the hemostatic properties of local wound dressings in the form of a sponge based on sodium alginate solutions in vivo and in vitro showed that the addition of a mixture of ultradispersed powders of micro- and nanoparticles of iron oxides with the prevalence of the epsilon phase led to an increase in the hemostatic properties of the dressings.

References

  1. Peng H.T. Hemostatic agents for prehospital hemorrhage control: a narrative review. Military Med. Res. 2020; 7 (1): 13. DOI: 10.1186/s40779-020-00241-z
  2. Hong Y., Zhou F., Hua Y., Zhang X., Ni C., Pan D. et al. A strongly adhesive hemostatic hydrogel for the repair of arterial and heart bleeds. Nat. Commun. 2019; 10 (1): 2060. DOI: 10.1038/s41467-019-10004-7
  3. Shabanova E.M., Drozdov A.S., Fakhardo A.F., Dudanov I.P., Kovalchuk M.S., Vinogradov V.V. Thrombin@Fe3O4 nanoparticles for use as a hemostatic agent in internal bleeding. Sci Rep. 2018; 8 (1): 233. DOI: 10.1038/s41598-017-18665-4
  4. Зейналов О.А., Комбарова С.П., Багров Д.В., Петросян М.А., Толибова Г.Х., Феофанов А.В. и др. О влиянии наночастиц оксидов металлов на физиологию живых организмов. Обзоры по клинической фармакологии и лекарственной терапии. 2016; 14 (3): 24–33. DOI: 10.17816/RCF14324-33
  5. Sanvicens N., Marco M.P. Multifunctional nanoparticles – properties and prospects for their use in human medicine. Trends Biotechnol. 2008; 8: 425–33. DOI: 10.1016/j.tibtech.2008.04.005
  6. Maldonado-Camargo L., Unni M., Rinaldi C. Magnetic characterization of iron oxide nanoparticles for biomedical applications. Methods Mol. Biol. 2017; 1570: 47–71. DOI: 10.1007/978-1-4939-6840-4_4
  7. Zhu N., Ji H., Yu P., Niu J., Farooq M.U., Akram M.W. et al. Surface modification of magnetic iron oxide nanoparticles. Nanomaterials (Basel). 2018; 8 (10): 810. DOI: 10.3390/nano8100810
  8. Барсукова Ю.Н., Мельникова О.А. Изучение высвобождения действующих веществ из гемостатического средства на гидрофильной основе. Journal of Siberian Medical Sciences. 2020; 1: 100–7. DOI: 10.31549/2542-1174-2020-1-100-107
  9. Липатова И.М., Юсова А.А., Гусев И.В. Свойства гидрогелей на основе смесей альгината натрия с другими полисахаридами природного происхождения. Chemistry of Plant Raw Material. 2014; 4: 59. DOI: 10.14258/jcprm.201404380
  10. Yang Q., Lei S. Alginate dressing application in hemostasis after using seldinger peripherally inserted central venous catheter in tumor patients. Indian J. Hematol. Blood. Transfus. 2015; 31 (4): 434–8. DOI: 10.1007/s12288-014-0490-1
  11. Миронов А.Н. (ред.) Руководство по проведению доклинических исследований лекарственных средств. Часть первая. М.: Гриф и К; 2012.
  12. Hemker H.C., Giesen P., Al Dieri R., Regnault V., de Smedt E., Wagenvoord R. et al. Calibrated automated thrombin generation measurement in clotting plasma. Pathophysiol. Haemost. Thromb. 2003; 33 (1): 4–15. DOI: 10.1159/000071636
  13. Sivkov A., Naiden E., Ivashutenko A., Shanenkov I. Plasma dynamic synthesis and obtaining ultrafine powders of iron oxides with high content of ε-Fe2O3. J. Magn. Magnet. Mat. 2016; 405: 158–68. DOI: 10.1016/j.jmmm.2015.12.072
  14. Shanenkov I., Sivkov A., Ivashutenko A., Medvedeva T., Shchetinin I. et al. High-energy plasma dynamic synthesis of multiphase iron oxides containing Fe3O4 and ε-Fe2O3 with possibility of controlling their phase composition. J. Alloys Comp. 2019; 774: 637–45. DOI: 10.1016/j.jallcom.2018.10.019
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  1. Peng H.T. Hemostatic agents for prehospital hemorrhage control: a narrative review. Military Med. Res. 2020; 7 (1): 13. DOI: 10.1186/s40779-020-00241-z
  2. Hong Y., Zhou F., Hua Y., Zhang X., Ni C., Pan D. et al. A strongly adhesive hemostatic hydrogel for the repair of arterial and heart bleeds. Nat. Commun. 2019; 10 (1): 2060. DOI: 10.1038/s41467-019-10004-7
  3. Shabanova E.M., Drozdov A.S., Fakhardo A.F., Dudanov I.P., Kovalchuk M.S., Vinogradov V.V. Thrombin@Fe3O4 nanoparticles for use as a hemostatic agent in internal bleeding. Sci Rep. 2018; 8 (1): 233. DOI: 10.1038/s41598-017-18665-4
  4. Zeynalov O.A., Kombarova S.P., Bagrov D.V., Petrosyan М.А., Tolibova G.H., Feofanov A.V. et al. About the influence of metal oxide nanoparticles on living organisms physiology. Reviews on Clinical Pharmacology and Drug Therapy. 2016; 14 (3): 24–33 (in Russ.). DOI: 10.17816/RCF14324-33
  5. Sanvicens N., Marco M.P. Multifunctional nanoparticles – properties and prospects for their use in human medicine. Trends Biotechnol. 2008; 8: 425–33. DOI: 10.1016/j.tibtech.2008.04.005
  6. Maldonado-Camargo L., Unni M., Rinaldi C. Magnetic characterization of iron oxide nanoparticles for biomedical applications. Methods Mol. Biol. 2017; 1570: 47–71. DOI: 10.1007/978-1-4939-6840-4_4
  7. Zhu N., Ji H., Yu P., Niu J., Farooq M.U., Akram M.W. et al. Surface modification of magnetic iron oxide nanoparticles. Nanomaterials (Basel). 2018; 8 (10): 810. DOI: 10.3390/nano8100810
  8. Barsukova Yu.N., Mel’nikova O.A. The study of the release of active substances from hemostatic agent on hydrophilic basis. Journal of Siberian Medical Sciences. 2020; 1: 100–7 (in Russ.). DOI: 10.31549/2542-1174- 2020-1-100-107]
  9. Lipatova I.M., Yusova A.A., Gusev I.V. Properties of hydrogels based on mixtures of sodium alginate with other naturally occurring polysaccharides. Chemistry of Plant Raw Material. 2014; 4: 59 (in Russ.). DOI: 10.14258/jcprm.201404380
  10. Yang Q., Lei S. Alginate dressing application in hemostasis after using seldinger peripherally inserted central venous catheter in tumor patients. Indian J. Hematol. Blood. Transfus. 2015; 31 (4): 434–8. DOI: 10.1007/s12288-014-0490-1
  11. Mironov A.N. (Ed.) About the guideline for the clinical trials of medicines. Part 1. Moscow; 2012 (in Russ.).
  12. Hemker H.C., Giesen P., Al Dieri R., Regnault V., de Smedt E., Wagenvoord R. et al. Calibrated automated thrombin generation measurement in clotting plasma. Pathophysiol. Haemost. Thromb. 2003; 33 (1): 4–15. DOI: 10.1159/000071636
  13. Sivkov A., Naiden E., Ivashutenko A., Shanenkov I. Plasma dynamic synthesis and obtaining ultrafine powders of iron oxides with high content of ε-Fe2O3. J. Magn. Magnet. Mat. 2016; 405: 158–68. DOI: 10.1016/j.jmmm.2015.12.072
  14. Shanenkov I., Sivkov A., Ivashutenko A., Medvedeva T., Shchetinin I. et al. High-energy plasma dynamic synthesis of multiphase iron oxides containing Fe3O4 and ε-Fe2O3 with possibility of controlling their phase composition. J. Alloys Comp. 2019; 774: 637–45. DOI: 10.1016/j.jallcom.2018.10.019

About Authors

  • Galina G. Belozerskaya, Dr. Med. Sc., Head of Laboratory of Pathology and Pharmacology of Hemostasis; ORCID
  • Andrey P. Momot, Dr. Med. Sc., Professor, Director; ORCID
  • Asaf R. Lempert, Cand. Med. Sc., Trainee-Researcher of Laboratory of Pathology and Pharmacology of a Hemostasis; ORCID
  • Dmitriy Yu. Bychichko, Junior Researcher, Doctor-Biochemist; ORCID
  • Valeriy A. Kabak, Manager of Laboratory of Pathology and Pharmacology of a Hemostasis; ORCID
  • Ol’ga E. Nevedrova, Cand. Biol. Sc., Senior Researcher; ORCID
  • Yulia S. Logvinova, Cand. Med. Sc., Researcher, Doctor-Biochemist; ORCID
  • Maksim S. Mironov, Laboratory Assistant; ORCID
  • Aleksandr A. Sivkov, Dr. Tech. Sc., Professor; ORCID
  • Ivan I. Shanenkov, Cand. Tech. Sc., Associate Professor; ORCID
  • ГEvgeniy M. Golubev, Head of Experimental and Production Department of Deep Processing of Plasma; ORCID
  • Tat’yana I. Shirokova, Deputy Head of Experimental and Production Department of Deep Processing of Plasma; ORCID
  • Marina V. Pykhteeva, Doctor-Laboratory Assistant of the Laboratory of Pathology of Hemostasis; ORCID

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