Clinical Physiology of Circulation

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


Development of new local hemostatic coverings based on kappa-carrageenan

Authors: D.Yu. Bychichko 1, O.E. Nevedrova 1, M.V. Pykhteeva 2, D.E. Belozerоv 2, G.G. Belozerskaya 1, A.P. Momot 3, V.A. Kabak 1, A.R. Lempert 1, L.S. Malykhina 1, M.S. Mironov 1, Yu.S. Logvinova 1, E.M. Golubev 1, T.I. Shirokova 1

Company:
1 National Medical Research Center for Hematology, Ministry of Health of the Russian Federation, Novyy Zykovskiy proezd, 4, Moscow, 125167, Russian Federation
2 Regional Clinical Hospital, ul. Lyapidevskogo, 1, korpus 2, Barnaul, 656045, Russian Federation
3 Altay Branch of the National Medical Research Center for Hematology, Ministry of Health of the Russian Federation, ul. Lyapidevskogo, 1, Barnaul, 656024, Russian Federation

E-mail: Сведения доступны для зарегистрированных пользователей.

DOI: https://doi.org/10.24022/1814-6910-2018-15-4-292-299

UDC: 615.472

Link: Clinical Physiology of Blood Circulaiton. 2018; 15 (4): 292-299

Quote as: Bychichko D.Yu., Nevedrova O.E., Pykhteeva M.V., Belozerov D.E., Belozerskaya G.G., Momot A.P., Kabak V.A., Lempert A.R., Malykhina L.S., Mironov M.S., Logvinova Yu.S., Golubev E.M., Shirokova T.I. Development of new local hemostatic coverings based on kappa-carrageenan. Clinical Physiology of Circulation. 2018; 15 (4): 292–9 (in Russ.). DOI: 10.24022/1814-6910-2018-15-4-292-299

Received / Accepted:  26.07.2018/18.09.2018

Full text:  

Abstract

Objective. Development of new hemostatic compositions of local action in the form of a sponge based on kappa-carrageenan.

Material and methods. 36 rabbits of the Chinchilla breed of both sexes weighing 3.0–4.5 kg were used as an experimental model. The rabbits were distributed in series and groups in accordance with the research tasks. The hemostatic activity of the samples was determined by the time of bleeding time and the volume of blood loss after the hemostatic compositions in the form of sponge were applied to the standard wound of rabbit liver by the method which described in the book “Preclinical Drug Research Guide”. Hemostatic activity of new samples in the form of a sponge based on kappa-carrageenan was researched. The influence of the mass fraction of kappa-carrageenan, solvents and pH of the initial solution on the hemostatic activity of new samples in the form of a sponge based on kappa-carrageenan has been estimated. The dependence of the time of formation of the fibrin clot on the contact surface of the sponge on the mass fraction of kappa-carrageenan and various solvents was determined based on the results of thromboelastometry.

Results. A comparative evaluation of the hemostatic activity of various samples in the form of a sponge based on kappacarrageenan showed that the best hemostatic effect was possessed by samples in the form of a sponge based on 1.5% and 2.0% kappa-carrageenan both at an acidic pH caused by the presence of 0.5% acetic acid, and at a neutral pH using distilled water as a solvent. Kappa-carrageenan-based local hemostatics in the form of a sponge produced from the solution with neutral pH would be recommended to reduce the risk of hemolysis in practical purposes.

Conclusion. The use samples of kappa-carrageenan-based in the form of sponge (obtained by new technologies) as
local hemostatic agents can stop massive local bleeding. The hemostatic activity of new samples depends on the choice
of solvent, pH changes in the initial solutions.

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About Authors

  • Bychichko Dmitriy Yur'evich, Junior Researcher, Doctor-Biochemist; orcid.org/0000-0003-1585-4415
  • Nevedrova Ol'ga Evgen'evna, Cand. Biol. Sc., Senior Researcher; orcid.org/0000-0001-9752-6647
  • Pykhteeva Marina Vladimirovna, Doctor-Laboratory assistant; orcid.org/0000-0003-4810-2922
  • Belozerov Dmitriy Evgen'evich, Doctor-Laboratory assistant; orcid.org/0000-0003-3433-7915
  • Belozerskaya Galina Gennad'evna, Dr. Med. Sc., Head of Laboratory of Pathology and Pharmacology of Hemostasis; orcid.org/0000-0001-8620-153X
  • Momot Andrey Pavlovich, Dr. Med. Sc., Professor, Director; orcid.org/0000-0002-8413-5484
  • Kabak Valeriy Alekseevich, Manager; orcid.org/0000-0002-3851-7510
  • Lempert Asaf Rudol'fovich, Postgraduate, Trainee-Researcher; orcid.org/0000-0002-6576-5712
  • Malykhina Larisa Sergeevna, Cand. Biol. Sc., Senior Researcher; orcid.org/0000-0002-6231-0069
  • Mironov Maksim Sergeevich, Laboratory Assistant; orcid.org/0000-0001-5230-7424
  • Logvinova Yuliya Sergeevna, Cand. Med. Sc., Researcher, Doctor-Biochemist; orcid.org/0000-0002-8392-1790
  • Golubev Evgeniy Mikhaylovich, Head of Experimental and Production Department of Deep Processing of Plasma; orcid.org/0000-0002-5405-8270
  • Shirokova Tat'ana Ivanovna, Deputy Head of Experimental and Production Department of Deep Processing of Plasma; orcid.org/0000-0002-2543-8071

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