Clinical Physiology of Circulation

ISSN 1814-6910 (Print)
ISSN 2410-9134 (Online)

 

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


Issue's catalogue ➤ КФК. 2020; 17 (1): 5-69 ➤ Новые перспективы исследования гемостатических покрытий на основе хитозана in vivo и in vitro

New prospects of research of hemostatic dressings based on chitosan in vivo and in vitro

Authors: G.G. Belozerskaya 1, A.P. Momot 2, M.V. Pykhteeva 3, V.А. Kabak 1, О.E. Nevedrova 1, D.Yu. Bychichko 1, А.R. Lempert 1, L.S. Malykhina 1, D.A. Momot 5, Е.M. Golubev 1, Т.I. Shirokova 1, M.S. Mironov 1, L.V. Akopyan 4

Company:
1 National Medical Research Center for Hematology, Moscow, 125167, Russian Federation
2 Altai Branch of the National Medical Research Center for Hematology, Barnaul, 656045, Russian Federation
3 Regional Clinical Hospital, Barnaul, 656045, Russian Federation
4 A.I. Yevdokimov Moscow State University of Medicine and Dentistry, Moscow, 127473, Russian Federation
5 Altai State Medical University, Barnaul, 656038, Russian Federation

E-mail: Сведения доступны для зарегистрированных пользователей.

DOI: https://doi.org/10.24022/1814-6910-2020-17-1-58-69

UDC: 616.12-089-092.9:620.3

Link: Clinical Physiology of Blood Circulaiton. 2020; 17 (1): 58-69

Quote as: Belozerskaya G.G., Momot A.P., Pykhteeva M.V., Kabak V.A., Nevedrova O.E., Bychichko D.Yu., Lempert A.R., Malykhina L.S., Momot D.A., Golubev E.M., Shirokova T.I., Mironov M.S., Akopyan L.V. New prospects of research of hemostatic dressings based on chitosan in vivo and in vitro. Clinical Physiology of Circulation. 2020; 17 (1): 58–69 (in Russ.). DOI: 10.24022/1814-6910-2020-17-1-58-69

Keywords:  hemostasis hemostatics of local action bleeding rabbits natural polymers thrombin generation test thromboelastometry chitosan

Received / Accepted:  07.11.2019/26.11.2019

Full text:  

Abstract

Objective. To conduct an experimental assessment of the hemostatic activity of local dressings in the form of a sponge based on chitosan solutions in vivo and in vitro.

Material and methods. For the production of the investigated dressings were used chitosan solutions with a mass fraction of 0.5, 1.0, 1.5 and 2.0% using 0.5% acetic or 0,5% lactic acids as solvents. On the basis of these solutions experimental samples of dressings in the form of a sponge were made. Hemostatic activity was determined by measuring of the time to stop bleeding and blood loss. The influence of the interaction of the contact surface of the dressings in the form of a sponge with blood was assessed by the results of counting the number of platelets and determining the level of fibrinogen in the blood plasma. An integral assessment of the hemostasis system was performed using thromboelastometry of blood stabilized with sodium citrate and thrombin generation test in blood plasma.

Results. High hemostatic activity in experiments in vivo had local sponge-based dressings based on 0.5 and 2.0% chitosan solutions in 0.5% acetic acid (67.65±24.23% and 65.65±9.68% respectively). The results of in vitrostudies of these dressings confirm the acceleration of the formation of a primary thrombus during their interaction with blood. Dressings based on all chitosan solutions in 0.5% lactic acid and 1,0 and 1.5% solutions of chitosan in 0.5% acetic acid did not show hemostatic activity in in vivo experiments and practically did not change the parameters of hemostasis in vitro.

Conclusion. The interconnection between the values of the hemostatic activity of wound dressings based on chitosan solutions in acetic acid in vivo during their local application to the wound surface of the rabbit's liver and researches of the system of hemostasis in vitro was revealed. Joint carrying out researches on studying of a system of a hemostasis both in vivo, and in vitro is represented the perspective direction for assessment of interrelation of a structural state and the mechanism of action of new hemostatics.

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About Authors

  • Galina G. Belozerskaya, Dr. Med. Sc., Head of Laboratory of Pathology and Pharmacology of Hemostasis; orcid.org/0000-0001-8620-153X
  • Andrey P. Momot, Dr. Med. Sc., Professor, Director of the Altai Branch; orcid.org/0000-0002-8413-5484
  • Marina V. Pykhteeva, Doctor-laboratory assistant; orcid.org/0000-0003-4810-2922
  • Valeriy A. Kabak, Manager; orcid.org/0000-0002-3851-7510
  • Ol'ga E. Nevedrova, Cand. Biol. Sc., Senior Researcher; orcid.org/0000-0001-9752-6647
  • Dmitriy Yu. Bychichko, Doctor-Biochemist, Junior Researcher; orcid.org/0000-0003-1585-4415
  • Asaf R. Lempert, Trainee-Researcher; orcid.org/0000-0002-6576-5712
  • Larisa S. Malykhina, Cand. Biol. Sc., Senior Researcher; orcid.org/0000-0002-6231-0069
  • Dmitriy A. Momot, Assistant of Chair of Faculty Therapy; orcid.org/0000-0002-7692-5048
  • Evgeniy M. Golubev, Head of Experimental and Production Department of Deep Processing of Plasma; orcid.org/0000-0002-5405-8270
  • Tat'yana I. Shirokova, Assistant Head of Experimental and Production Department of Deep Processing of Plasma; orcid.org/0000-0002-2543-8071
  • Maksim S. Mironov, Laboratory Assistant; orcid.org/0000-0001-5230-7424
  • Lyudmila V. Akopyan, Cand. Med. Sc., Assistant of Chair of Otorhinolaryngology; orcid.org/0000-0002-4328-0001

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