Clinical Physiology of Circulation

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


Studying the properties of the film compositions with gelatin and colchicine

Authors: L.A. Bockeria, O.L. Bockeria, S.P. Novikova, R.R. Salokhedinova, L.N. Nikolashina, O.V. Shustrova, V.S. Sivtsev

Company:
A.N. Bakoulev Scientific Center for Cardiovascular Surgery, Rublevskoe shosse, 135, Moscow, 121552, Russian Federation

E-mail: Сведения доступны для зарегистрированных пользователей.

Link: Clinical Physiology of Blood Circulaiton. 2014; (): -

Quote as: Bockeria L.A., Bockeria O.L., Novikova S.P., Salokhedinova R.R., Nikolashina L.N., Shustrova O.V., Sivtsev V.S. Studying the properties of the film compositions with gelatin and colchicine. Klinicheskaya Fiziologiya Krovoobrashcheniya. 2014; 3: 57-66.

Full text:  

Abstract

Objective – to assess the possibility of creating a film compositions based on natural biodegradable polymer – gelatin and colchicine.
Material and methods. To obtain film compositions using natural polymer – gelatin. As the biologically active substance – herbal drug with anti-inflammatory properties – colchicine, a plasticizer – glycerin, a crosslinking agent – glutaraldehyde.
Evaluated: the strength characteristics of film compositions, the timing of their hydrolytic degradation, desorption of colchicine compositions, sterilization effect.
Results. Shows a linear dependence of the amount allocated from the amount of the immobilized colchicine. Active rapid release unbound colchicine occurs in the first 30 minutes – released from 65 to 75% colchicine depending on its amount in the composition. Adding to the film composition colchicine loosens its structure – the strength is reduced by 18%. Hydrolytic degradation film compositions with colchicine is faster. Sterilization increases the amount of liberated colchicine at 8% and hardens gelatin film with colchicine at 15%.
Conclusion. The possibility of obtaining compositions based on gelatin-immobilized colchicine in different proportions. Studied their physico-chemical, physico-mechanical properties. The received compositions have the prolonged allocation of colchicine, adjustable terms of the hydrolytic destruction, necessary complex of mechanical properties. The bulk of the injected unbound colchicine released from the film compositions quickly in the first 30 minutes. The prolonged release of colchicine in small doses as shown in the work provided by the remaining biodegradable gelatin complex with colchicine. It can be assumed that the therapeutic effect of the compositions can be associated not so much with the amount of injected colchicine as a prolongation of its isolation in small doses including and biodegradation of the complex. It is advisable to pay attention in further biological studies film compositions in experiments on animals.

References

1. Григорьева М.В. Полимерные системы с контролируе- мым высвобождением биологически активных соедине- ний. Биотехнология. 2011; 4 (2): 9–19.
2. Ананьев В.Н. Нанотехнологическая матрица механизма действия и доставки лекарственных препаратов в виде же- латиновых пленок. Фармацевтические науки. 2011; 5: 53–7.
3. Фурин В.А. Разработка методов применения лекарствен- ных желатиновых пленок в военной и гражданской меди- цине: Автореф. дис. ... канд. мед. наук. Уфа; 2004.
4. Шурыгина И.А., Шурыгин М.Г. Лекарственная пленка пролонгированного действия, способ изготовления и способ ее применения. Патент RU, 02445074 С1; 2010.
5. Новиков Ю.Т., Фурин В.А., Сулычева Н.Ю., Куличенко Е.Н., Ананьев В.Н. Гомеопатическая композиция. Патент RU, 2192846; 2002.
6. Мизина П.Г. и др. Способ получения лекарственной фи- топленки. Патент RU, 2155071; 2000.
7. Бокерия Л.А., Новикова С.П. Биорезорбируемая гидроге- левая полимерная композиция с биологически активны- ми веществами (варианты). Патент RU, 2519103; 2014.
8. Yoshioka I., Saiki Y., Sakuma K., Iguchi A., Moriya T., Ika- da Y., Tabayashi K. Bioabsorbable gelatin sheets latticed with polyglycolic acid can eliminate pericardial adhesion. Ann. Thorac. Surg. 2007; 84: 864–70.
9. Tsujimoto H., Tanzawa A., Matoba M., Hashimoto A., Suzu- ki S., Morita S. et al. The anti-adhesive effect of thermally cross-linked gelatin film and its influence on the intestinal anastomosis in canine models. J. Biomed. Mater. Research. Part B: Applied Biomaterials. 2013; 101 (1): 99–109.
10. Бокерия Л.А., Новикова С.П. Протезы кровеносных со- судов и кардиохирургические заплаты с тромборезис- тентными, антимикробными свойствами и нулевой хи- рургической пористостью. Бюллетень НЦССХ им. А.Н. Ба- кулева РАМН. 2008; 9 (4): 5–20.
11. Новикова С.П., Лосева С.В., Салохединова Р.Р., По- пов Д.А., Анучина Н.М. Комплексная оценка стабильно- сти свойств медицинских изделий «БАСЭКС» при дли- тельных сроках хранения. Клиническая физиология кровообращения. 2011; 1: 76–81.
12. Бокерия Л.А., Новикова С.П., Лосева С.В., Шустро- ва О.В. Способ обработки синтетических текстильных имплантируемых медицинских изделий, контактирую- щих с кровью. Патент RU, 2462273; 2012.
13. Новикова С.П., Салохединова Р.Р., Лосева С.В., Никола- шина Л.Н., Левкина А.Ю. Анализ физико-механических и структурных характеристик протезов кровеносных со- судов. Грудная и сердечно-сосудистая хирургия. 2012; 4: 27–33.
14. Кудрявцева Ю.А., Насонова М.В., Журавлева И.Ю. По- слеоперационные спайки в кардиохирургии: проблемы и решения. Патология кровообращения и кардиохирургия. 2011; 1: 100–4.
15. Sakuma K., Iguchi A., Ikada Y., Tabayashi K. Closure of the pericardium using synthetic bioabsorbable polymers. Ann. Thorac. Surg. 2005; 80: 1835–40.
16. Bel A., Kachatryan L., Bruneval P., Peyrard S., Gagnieu C., Fabiani J.N. et al. A new absorbable collagen membrane to reduce adhesions in cardiac surgery. Interact. CardioVasc. Thorac. Surg. 2010; 10(2): 213–6.
17. Zhou J., Liwski R.S., Elson C., Lee T.D. Reduction in post- surgical adhesion formation after cardiac surgery by applica- tion of N,O-carboxymethyl chitosan. J. Thorac. Cardiovasc. Surg. 2010; 140 (4): 801–6.
18. Imazio M., Belli R., Brucato A., Ferrazzi P., Patrini D., Martinelli L. et al. Rationale and design of the colchicine for Prevention of the Post-pericardiotomy Syndrome and Post- operative Atrial Fibrillation (COPPS-2 trial): a randomized, placebo-controlled, multicenter study on the use of colchicine for the primary prevention of the postpericardiotomy syn- drome, postoperative effusions, and postoperative atrial fibril- lation. Am. Heart J. 2013; 166 (1): 13–9.
19. Mack D.R., Cahoon W.D. Jr, Lowe D.K. Colchicine for the primary prevention of the postpericardiotomy syndrome. Ann. Pharmacother. 2011; 45 (6):803–6.
20. Kuo I.F., Pearson G.J., Koshman S.L. Colchicine for the pri- mary and secondary prevention of pericarditis: an update. Ann. Pharmacother. 2009; 43 (12): 2075–81.
21. Алекберова З.С., Барскова В.Г. Колхицин в ревматоло- гии – вчера и сегодня. Будет ли завтра? Современная рев- матология. 2010; 2: 25–9.
22. Deftereos S., Giannopoulos G., Papoutsidakis N., Panago- poulou V., Kossyvakis Ch., Raisakis K. et al. Colchicine and the heart: pushing the envelope. J. Am. Coll. Cardiol. 2013; 62 (20): 1817–25.
23. Little A., Tung D., Truong C., Lapinsky S., Burry L. Colchicine overdose with coingestion of nonsteroidal antiin- flammatory drugs. CJEM. 2014; 16 (3): 252–6.
24. Gradus-Pizlo I., Wilensky R.L., March K.L., Fineberg N., Michaels M., Sandusky G.E. et al. Local delivery of biodegradable microparticles containing colchicine or a colchicine analogue: effects on restenosis and implications for catheter-based drug delivery. J. Am. Coll. Cardiol. 1995; 26 (6): 1549–57.
25. Эпштейн О.И. Регуляторные возможности сверхмалых доз. Бюллетень экспериментальной биологии и медицины. 2002; Приложение 4: 8–14.

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