Clinical Physiology of Circulation

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, Director of Bakoulev National Medical Research Center for Cardiovascular Surgery

New opportunities for a comprehensive assessment of the hemostatic properties of local dressings in vivo and in vitro

Authors: Kabak V.А.1, Belozerskaya G.G.1, Momot A.P. 2, Pykhteeva M.V. 3, Nevedrova О.E.1, Bychichko D.Yu.1, Lempert А.R.1, Malykhina L.S.1, Momot D.A.4, Golubev Е.M.1, Shirokova Т.I.1, Mironov M.S.1, Kuleshova S.B. 1

Company:
1 National Medical Research Center for Hematology, Moscow, 125167, Russian Federation
2 Altai Branch of the National Medical Research Center for Hematology, Barnaul, 656045, Russian Federation
3 Regional Clinical Hospital, Barnaul, 656045, Russian Federation
4 Altai State Medical University, Barnaul, 656038, Russian Federation

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DOI: https://doi.org/10.24022/1814-6910-2020-17-2-121-129

UDC: 577.1

Link: Clinical Physiology of Blood Circulaiton. 2020; 17 (2): 121-129

Quote as: Kabak V.A., Belozerskaya G.G., Momot A.P., Pykhteeva M.V., Nevedrova O.E., Bychichko D.Yu., Lempert A.R., Malykhina L.S., Momot D.A., Golubev E.M., Shirokova T.I., Mironov M.S., Kuleshova S.B. New opportunities for a comprehensive assessment of the hemostatic properties of local dressings in vivo and in vitro. Clinical Physiology of Circulation. 2020; 17 (2): 121–9 (in Russ.). DOI: 10.24022/1814-6910-2020-17-2-121-129

Received / Accepted:  28.11.2019/25.12.2019

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Abstract

Objective. Conduct a comprehensive analysis of the hemostatic properties of local wound dressings in experiments in vivo and using a new test-system in vitro.

Material and methods.Solutions of sodium alginate with a weight fraction of 0.5 and 2.0% in distilled water, chitosan with a weight fraction of 0.5 and 2.0%, using 0.5% acetic acid as solvent and 0.5 and 2.0% aqueous kappa-carrageenan gels were used for preparing the test dressings. Experimental samples of sponge-shaped dressings were prepared from these solutions. The hemostatic activity of the samples was determined by measuring bleeding stop time and blood loss volume. The effect of the interaction of the contact surface of sponge-shaped dressings with blood in vitro was evaluated by platelet count and blood plasma fibrinogen level determination. An integral evaluation of the hemostasis system was performed by thromboelastometry and thrombin generation test.

Results.Sponge-shaped dressings based on 2.0% aqueous sodium alginate solution, 2.0% aqueous kappa-carrageenan gel and 0.5, 2.0% chitosan solutions in 0.5% acetic acid showed high values of hemostatic activity in experiments in vivo. By the analysis of the results of the main substrates of blood coagulation, thromboelastometry and thrombin generation test, the data obtained from in vitro experiments using the new test system confirmed the process of accelerated thrombus formation.

Conclusion. Complex analysis of hemostatic properties of local wound sponge-shaped dressings based on polymers in vivo and in vitro allows reliable and comprehensive evaluation of their effectiveness. The results obtained using the new test system in studies in vitro and such experimental data in vivo as blood stopping time and bleeding rate, blood loss volume, adhesive ability of the dressings determine the optimal choice of local hemostatic.

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About Authors

  • Valeriy A. Kabak, Manager; orcid.org/0000-0002-3851-7510
  • Galina G. Belozerskaya, Dr. Med. Sc., Head of Laboratory of Pathology and Pharmacology of Hemostasis; orcid.org/0000-0001-8620-153X
  • Andrey P. Momot, Dr. Med. Sc., Professor, Director of the Altai Branch; orcid.org/0000-0002-8413-5484
  • Marina V. Pykhteeva, Doctor – Laboratory Assistant; orcid.org/0000-0003-4810-2922
  • Ol'ga E. Nevedrova, Cand. Biol. Sc., Senior Researcher; orcid.org/0000-0001-9752-6647
  • Dmitriy Yu. Bychichko, Doctor-Biochemist, Junior Researcher; orcid.org/0000-0003-1585-4415
  • Asaf R. Lempert, Trainee-Researcher; orcid.org/0000-0002-6576-5712
  • Larisa S. Malykhina, Cand. Biol. Sc., Senior Researcher; orcid.org/0000-0002-6231-0069
  • Dmitriy A. Momot, Assistant of Chair of Faculty Therapy; orcid.org/0000-0002-7692-5048
  • Evgeniy M. Golubev, Head of Experimental and Production Department of Deep Processing of Plasma; orcid.org/0000-0002-5405-8270
  • Tat'yana I. Shirokova, Assistant Head of Experimental and Production Department of Deep Processing of Plasma; orcid.org/0000-0002-2543-8071
  • Maksim S. Mironov, Laboratory Assistant; orcid.org/0000-0001-5230-7424
  • Svetlana B. Kuleshova, Head of Laboratory of Bioclinic and Experimental Animals; orcid.org/0000-0002-6280-0834

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